The relationship and significance of echocardiographic indexes of acute myocardial infarction with serum heart-type fatty acid binding protein and chitinase-3 like protein-1 / 中国医师进修杂志

Objective:To investigate the relationship between left ventricular ejection fraction (LVEF), left ventricular end diastolic diameter (LVEDD), wall motion score (WMS) of echocardiographic indexes and serum heart type fatty acid binding protein (h-FABP), chitinase-3 like protein-1 (YKL-40) and their predictive value for prognosis in patients with acute myocardial infarction (AMI).Methods:A retrospective selection of 130 AMI patients admitted from January 2020 to March 2021 in the University of Hong Kong Shenzhen Hospital were selected as the research objects. According to whether major adverse cardiovascular events or death occurred within 30 d, they were divided into a good prognosis group (85 cases) and a poor prognosis group (45 cases). The echocardiographic indexes, serum h-FABP and YKL-40 levels were compared between the two groups, the correlation between serum indexes and color Doppler ultrasound indexes and the value of echocardiographic indexes for prognosis were also analyzed.Results:When the poor prognosis group was admitted to the hospital and after 1 week of treatment, the LVEF was lower than that in good prognosis group: (40.12±4.10)% vs. (44.37±5.06)% and (47.62±4.83)% vs. (53.29±5.31)%; the LVEDD, WMS, serum h-FABP and YKL-40 level were higher than those in good prognosis group: (52.78±3.67) mm vs. (49.16±3.14) mm and (48.35±4.08) mm vs. (44.20±3.72) mm, (23.42±3.11) scores vs. (20.09±2.87) scores and (20.07±3.70) scores vs. (15.20±2.34) scores, (28.76±4.22) mg/L vs. (22.35±3.19) mg/L and (20.04±3.33) mg/L vs. (14.20±2.76) mg/L, (40.17±5.10) μg/L vs. (34.83±3.25) μg/L and (32.09±4.38) μg/L vs. (27.20±3.07) μg/L, and there were statistical differences ( P<0.05). LVEF was negatively correlated with serum h-FABP and YKL-40 neither at admission nor 1 week after treatment, and LVEDD and WMS were positively correlated with serum h-FABP and YKL-40 ( P<0.05). The areas under the curve for combined prediction of prognosis by LVEF, LVEDD, and WMS was the largest at 0.873. The survival rates of high-risk patients indicated with LVEF, LVEDD, and WMS were lower than that of low-risk patients: 56.52% vs. 90.91%, 52.38% vs. 91.67%, and 54.17% vs. 95.24%, and there were statistical differences ( P<0.05). Conclusions:The echocardiographic parameters LVEF, LVEDD and WMS in patients with AMI are closely related to serum h-FABP and YKL-40, which can provide an objective basis for the evaluation of myocardial pathophysiological changes. Moreover, the combined detection of LVEF, LVEDD, and WMS can be used to further assess the prognostic survival status, with a positive significance for guiding clinical treatment plans.

Application of various genetic techniques for the diagnosis of Prader-Willi syndrome / 中华医学遗传学杂志

OBJECTIVE@#To discuss the advantages and technical limitations of various molecular genetic techniques in the diagnosis of two infants featuring all-round developmental retardation.@*METHODS@#The two patients were initially screened by using chromosomal microarray analysis (CMA). For patient 1, his parents were also subjected to CMA analysis, and the data was analyzed by using ChAS and UPD-tool software. For patient 2, methylation-specific PCR (MS-PCR) was carried out.@*RESULTS@#Patient 1 was diagnosed with maternal uniparental disomy (UPD) type Prader-Willi syndrome (PWS) by CMA and UPD-tool family analysis. His chromosomes 15 were of maternal UPD with homology/heterology. Patient 2 was diagnosed with deletion type PWS by combined CMA and MS-PCR.@*CONCLUSION@#Correct selection of laboratory methods based on the advantages and limitations of various molecular techniques can help with diagnosis of genomic imprinting disorders and enable better treatment and prognosis through early intervention.

Quantitative assessment of stroke patients based on dynamic time warping algorithm / 生物医学工程学杂志

In order to accurately evaluate the similarity of motions during daily rehabilitation training for stroke patients, this paper proposed a novel quantitative assessment method based on dynamic time warping (DTW) algorithm. Firstly, the raw accelerometer signals were preprocessed to eliminate the noise. Secondly, the similarity between the accelerometer signals and four standard task templates was calculated respectively, and then the motion was recognized based on the similarity measurements. Finally, the corresponding quantitative assessment model was used to compute the result. The clinical experimental results showed that there were significant differences in the shortest path distance ( value) of DTW between different tasks, and the classification accuracy could be up to 91% when the value was selected as the classification feature. Additionally, with the process of rehabilitation, the value decreased gradually, which means that the value can be taken as the assessment index to evaluate the quality of designated tasks for stroke patients. It also indicated that the value could be applied into the scene of automatic prescription generation and interactive gaming to determine whether it is needed to change the rehabilitation plan or adjust the game difficulty level, so as to implement the individualized rehabilitation services.

Effect of all-trans-retinoic acid on proliferation of human lung adenocarcinoma cell line A549 and expression of APLNR gene / 临床检验杂志

Objective To explore the effects of all-trans-retinoic acid (ATRA) on the proliferation of human lung adenocarcinoma cell line A549 and the expression of APLNR (apelin receptor) gene.Methods The inhibition of proliferation of human lung adenocarcinoma cell lines A549 cultured in vitro with or without ATRA was measured by MTT (methyl thiazolyl tetrazolium,MTT) method.The morphological changes in the cells were observed by light microscopy.The cell cycle and apoptosis were analyzed by flow cytometry.The levels of APLNR,cyclin D1 and p16 proteins were detected by western blot.Results After treatment of ATRA,the proliferation of A549 cells was obviously inhibited in dose-and time-independent manner (P ＜ 0.01).The cell morphology was significantly changed.The cycle of A549 cells was blocked at G0/G1 phase and the apoptosis rate was increased.With the increasing concentration of ATRA,the expressions of cyclin D1 and APLNR were down-regulated but the expression of p16 was up-regulated (P ＜ 0.01).Conclusion ATRA could inhibit the proliferation of A549 cells by retardant cell cycle of A549 cells at G0/G1 phase and inducing the apoptosis,and down-regulate the expression of APLNR gene.

Study on pharyngeal wall floppiness of patients with obstructive sleep apnea-hypopnea syndrome attributable to non-structural factors / 中华耳鼻咽喉头颈外科杂志

Objective@#Acoustic pharyngealmetry technology is utilized to evaluate the change and clinical significance of obstructive sleep apnea-hypopnea syndrome (OSAHS) patients caused by non-upper airway structural factor and normal individuals′ PWF(pharyngeal wall floppiness).@*Methods@#Acoustic pharyngealmetry instrument of Ecconvision was utilized to examine 102 OSAHS patients and 50 normal individuals, separately recorded their volume of pharyngeal cavity in sit or supine position, calculated PWF in sit or supine position, and SPSS 12.0 of tware was used to analyze data.@*Results@#PWF was 0.14±0.09 in sit position and PWF was 0.21±0.10, (t=5.96, t=9.63, P<0.001)in supine position of OSAHS group, which were all significantly higher than those of control group. PWFs in supine position of OSAHS group and control group were evidently higher than PWF(t=-11.91, P<0.001; t=-2.32, P=0.025) in sit position. ΔPWF(PWF_supine-PWF_sit)was 0.063±0.054 in OSAHS group which was significantly greater than in control(F=41.173, P<0.01). PWF in sit position and supine position were all positively related with age(r=0.714, r=0.735, P<0.001)while irrelevant with BMI(P>0.05).@*Conclusions@#PWF can be utilized to be an index to reflect the physiological feature of upper airway. PWF can more precisely reflect upper airway collapsibility of OSAHS patients on the condition of PWF in supine position. Pharyngeal wall floppiness quantified as a high PWF index is a non-structure vital factor of OSAHS patients and plays a role of guiding us to make personal treatment plans for OSAHS patients.

Relationship between anoikis and tumor invasion and metastasis / 肿瘤研究与临床

Anoikis,a specific form of programmed cell death,is triggered by cell detachment from extracellular matrix or adjacent cell.Studies have found that the most kinds of tumor cells had anoikis resistance characteristic,which may inhibit pro-apoptosis protein,block anoikis inside and outside pathways,up-regulate pro-survival factor signals,and promote survival,invasion and metastasis of tumor cells in the end.The review summarized the mechanism of anoikis and the relationship between anoikis and tumor invasion and the metastasis.

Multivariate analysis of prognostic makers in patients with nasopharyngeal carcinoma intreatment of radiotherapy combined with induction chemotherapy / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To observe the therapeutic effect of radiotherapy combined with TPF in patients with nasopharyngeal carcinoma (NPC) and to explore the influencing factors on prognosis of NPC.@*METHOD@#A retrospective analysis of 144 patients with nasopharyngeal carcinoma receiving radiotherapy combined with chemotherapy, from January 2006 to December 2011, was conducted. The survival analysis in patients with nasopharyngeal carcinoma were performed. The impact of age, sex, clinical stage, alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), lactate dehydrogenase (LDH), homocysteine (Hcy), uric acid, NK cell proportion, T cell proportion, Carbohydrate antigen on survival time was observed with univariate and multivariate methods.@*RESULT@#By the last follow-up, 9 cases were lost . The follow-up rate was 93.75 percent and the average follow-up time were 54.67 ±17.17 (37-88) months. The age, clinical stage, LDH, proportion of T cells and NK cells were independent factor in patients with NPC with univariate analysis (P < 0.01). COX regression multivariate analysis revealed that age, clinical stage and LDH were strong risk factor affecting prognosis (P < 0.01) and the proportion of NK cells and T cells were protective factors (P < 0.01).@*CONCLUSION@#Radiotherapy combined with TPF is more effective than radiotherapy alone in patients with nasopharyngeal carcinoma . Age, clinical stage and LDH may adversely influence the disease prognosis, and NK cells and T cell proportion may act as protective factors.

The reference values and Z scores regression equations of normal newborns undergoing echocardiography / 中华医学超声杂志（电子版）

Objective To investigate the reference values and Z scores regression equations of newborn undergoing echocardiography. Methods Two hundred and eighty-eight newborns (aged 0-28 days) of Shenzhen Children′s Hospital underwent echocardiography examination, including M-mode, two-dimensional (2D) and real-time three-dimensional (3D) echocardiography, color Doppler lfow imaging (CDFI) and tissue Doppler imaging. The correlation between echocardiography results and weight were analyzed and Z scores were calculated. Results The normal values of right ventricular diameter (RV) and left ventricular end-diastolic diameter (LVEDD) measured by M-mode, the mitral annulus diameter in four chamber view (MV-D1), mitral annulus diameter in two chamber view (MV-D2), mitral annulus diameter in longitudinal view (MV-D3), aortic ring diameter (ARD), aortic sinus diameter (ASD), ascending aorta diameter (AAO), transverse aorta diameter (TA), aortic isthmus diameter (AI), aorta diaphragm diameter (AO-Dia), tricuspid annulus diameter in four chamber view (TV-D1), tricuspid annulus diameter in right ventricular inlfow tract view (TV-D2), right ventricular outlfow tract diameter (RVOT), pulmonary valve diameter (PVD) and main pulmonary artery diameter (PA) measured by 2D echocardiography and the normal values of mitral valve inflow Doppler component during early diastole (MV-E), mitral valve inlfow Doppler component during atrial contraction (MV-A), tricuspid valve inlfow Doppler component during early diastole (TV-E), tricuspid valve inflow Doppler component during atrial contraction (TV-A), aortic valve peak velocity (AV-max), aortic valve velocity-time integral (AV-VTI), pulmonary valve peak velocity (PV-max), pulmonary valve velocity-time integral (PV-VTI) measured by pulse Doppler, the mitral annular tissue Doppler component during systole (MV-s′), mitral annular tissue Doppler component during early diastole (MV-e′), mitral annular tissue Doppler component during atrial contraction (MV-a′), tricuspid annular tissue Doppler component during systole (TV-s′), tricuspid annular tissue Doppler component during early diastole (TV-e′), tricuspid annular tissue Doppler component during atrial contraction (TV-a′), interventricular septum annular tissue Doppler component during systole (IVS-s′), interventricular septum annular tissue Doppler component during early diastole (IVS-e′), interventricular septum annular tissue Doppler component during atrial contraction (IVS-a′) measured by tissue Doppler, the normal values of left atrial volume (LAV), left ventricular end-systolic volume (LVEDV), stroke volume (SV) and cardiac output (CO) measured by bi-plane method and the normal values of LVEDV, SV and CO measured by real-time tri-plane method, together with the normal values of left ventricular (LV) mass, left ventricular mass index [LV mass/BSA, LV mass/H2.7, body surface area (BSA) and height (H)], all showed nonlinear positive correlations with body weight (all P0.05). Except for RV, MV-D1, MV-D2, MV-D3, TV-D1, TV-E, MV-s′, IVS-a′, TV-s′and TV-e′, all R2 obtained by nonlinear regression method (lnY=a+bX+cX2+dX3) were larger than those obtained by linear regression method (Y=a+bX). The Z score showed a normal distribution and no correlation with body weight. Conclusions The normal reference values of newborn undergoing echocardiography reflect the variation in weight. The Z scores can be obtained by the predicted nonlinear regression equations and show standard normal distribution. The echocardiography normal reference values have important significance for the diagnosis and treatment of neonatal heart disease.

A comparative study on normal fear of Chinese adolescents between urban and rural areas / 中华行为医学与脑科学杂志

Objective To compare the features of adolescents' normal fear between urban and rural areas in China. Methods 648 Urban and 478 rural adolescents were investigated with the revised Fear Survey Schedule For Children -II . Results (1)The most common fear of adolescents in urban and rural areas was very same,namely taking dangerous drugs,someone in my family having an accident,not being able to breath,someone in my family dying,nuclear war,my parents separating or getting divorced,being kidnapped,but only one item was not same ,namely urban adolescents fear having no friends ,rural adolescents fear AIDS. (2)The amount of fear items( t =11.42,P <0.01) and the degree of general fear (urban =1.62±0.28,rural =1.83±0.26,t =12.72,P <0.01) ,each dimension fear(P <0.01) of rural adolescents were significantly higher than that of urban adolescents.(3)The degree of fear about danger and injure( F =85.46,P <0.01),animal (female=1.84±0.47,male=1.54±0.45,F =156.86,P <0.01),social relationship ( F =32.23,P <0.01)of female adolescents were significantly higher than that of male adolescents.(4) In all adolescents ,the degree of the general fears( F =13.781,P <0.01) danger and injure fears( F =17.164,,P <0.01) decreased gradually as they grew up ,and the degree of unknown and uncertainty fear(( F =5.01,P <0.01),social fear ( F =4.42,P <0.05)of middle school students were higher than that of high school students; urban middle school students were significantly higher than that of urban high school students in failure and punishment fear.Conclusion (1)There are significant consistency on the most normal fears,the trend of development about the general fears,danger and injure fears,unknown and uncertainty fear,social fear for adolescents between urban and rural areas .(2)The amount of fear items and the degree of general fear ,each dimension fear of school-aged rural adolescents were significantly higher than that of urban adolescents;and there isn't common trend of development on the failure and punishment fear for rural and urban adolescents.

Evaluation of left ventricular function in children with Kawasaki disease by Tei index of adenosine triphosphate-atropine stress echocardiography / 中华超声影像学杂志

R prognosticate the lesion of coronary artery in KD has influence on left ventricular function.

Autologous bone-marrow mononuclear cells transplantation in experimental acute myocardial infarction / 中国康复理论与实践

@#ObjectiveTo investigate the survival and differentiation of autologous bone-marrow mononuclear cell (ABM-MNCs) after transplanted to infarcted area and border area, and the effect of ABM-MNC on the cardiac function.Methods40 male big-ear Japanese rabbits were divided randomly into the transplanted group and control group with 20 animals in each group. Acute myocardial infarction model was made by ligating left anterior descending artery. 7 days later, Brdu labeled ABM-MNCs were injected into myocardium in the transplanted group, while the control rabbits were injected with saline. Six weeks later, tests of histology and immunohistochemistry were performed.ResultsViable cells labeled with Brdu can be identified in the infarcted area, and myocytes and endothelial cells labeled with Brdu can also be found in the border area, these cells demonstrated myogenic differentiation with the expression of α-actin by immunostaining. While, no cells labeled with Brdu were found in the control group. Moreover, the vessel density of the transplanted group in the borders of the infarction was higher than the control group (P<0.05), but there was no difference in infarcted area between two groups (P>0.05).At the 6 weeks after experiment, the cardiac function was improved in both groups, but there was a significant difference between two groups (P<0.05).ConclusionABM-MNCs injected into the infarcted myocardium can survive in both the infarcted and border areas, and differentiate into endothelial cells and other cells which are able to obtain the characters of myocytes, and increase the vessel density in border area, improve the cardiac function.